\[\frac{(a - 4b)^{2}}{\text{ab}} - \frac{(a + 4b)^{2}}{\text{ab}} =\]
\[= \frac{a^{2} - 8ab + 16b^{2} - a^{2} - 8ab - 16b^{2}}{\text{ab}} =\]
\[= - \frac{16ab}{\text{ab}} = - 16.\]